Xenbase Image ID: 147936

Figure 6. The effect of Xdd1 on cell movements may depend on cell–cell interactions. (a)Xdd1 inhibits morphogenetic movements of the dorsal marginal zone. Dorsal marginal zone explants were isolated at stage 10 from embryos that had been injected earlier with 1 ng of Xdd1 mRNA (on the left) or Xdd2 mRNA (on the right). (b,c) Lineage tracing of Xdd1-microinjected embryos. (b) Embryos were injected at eight-cell stage into the dorso-animal blastomere with β-gal mRNA only (bottom embryo) or with β-gal mRNA plus Xdd1 mRNA (two top embryos). (c) Two top dorso-animal blastomeres at the 32-cell stage were injected with 5 nl of β-gal RNA (two top embryos) or Xdd1 plus β-gal RNAs (three bottom embryos). The arrowhead points to the area in which developmental abnormalities are observed, despite the absence of Xdd1 and β-gal RNA (unstained). Embryos were cultured until stage 35, then fixed and β-gal-expressing cells were visualized histochemically. Image published in: Sokol SY (1996) Copyright © 1996. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page