Xenbase Image ID: 147951

Fig. S2. Specificity of Irx morpholinos. To test the specificity of the morpholinos (MOs), we generated Xenopus laevis Irx constructs bearing a Myc epitope either at the N (MT-Irx) or at the C terminus (Irx-MT). MOs are antisense oligonucleotides that block translation efficiently only if their target sequence is within 25 bases of the translation start site. This is the case for Irx-MT mRNA but not for the MT-Irx transcripts. (A,D,G,J,M) Myc staining of stage 12 embryos injected with 0.5 ng of MT-Irx mRNAs and 10 ng of the corresponding MO. The MOs are unable to block MT-Irx translation. (B,E,H,K,N) Myc staining in stage 12 embryos injected with 0.5 ng of Irx-MT mRNAs. (C,F,I,L,O) Myc staining in stage 12 embryos injected with 0.5 ng of Irx-MT mRNAs and 10 ng of the corresponding MO. Each MO effectively blocks the translation of its corresponding Irx-MT mRNA. The different MOs can only block the translation of their corresponding genes (not shown). Image published in: Alarcón P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page