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Fig. S3. Irx2, Irx4 and Irx5 are not required for kidney formation. All embryos were injected with specific Irx MOs and GFP mRNA in V2.2 blastomere of 8- to 16-cell stage embryos. Stage 22 embryos injected and triple labelled for Sox2 and Lim1 in purple and 12/101 in brown. (A,D,G) Control uninjected sides. (B,E,H) Morpholino-injected sides. Insets show the GFP distribution in the injected embryos. Impairment of Irx2, Irx4 or Irx5 does not affect Lim1 expression. Transverse sections of injected embryos are shown in C,F,I. Image published in: Alarcón P et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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