Xenbase Image ID: 148042

Figure 4. Requirement of FoxM1-dependent G2-M progression for neuronal differentiation. (A) Xenopus embryos were analyzed for Cdc25B and cyclin B3 by RT-PCR. (B) Embryos pre-injected with control MO or Cdc25B-MO (18 ng) at the one-cell stage were cultured until st. 32 and photographed to show morphological phenotypes. (C) Embryos pre-injected with both lacZ mRNA (100 pg) and control MO or Cdc25B-MO (18 ng) at one blastomere at the two-cell stage were fixed at st. 13, immunostained for pH3, and analyzed as in Fig. 1B. *P<0.01. (D) Embryos were co-injected with lacZ mRNA (100 pg) and either control MO or Cdc25B-MO (18 ng) at one blastomere at the two-cell stage, cultured until st. 14, and analyzed by WISH. In each panel, the injected side (β-Gal, red) of the embryo is on the right (dorsal view, anterior up). (E) Animal caps from the late blastula embryos pre-injected with control MO or Cdc25B-MO (36 ng) at the one-cell stage were cultured with or without Activin (200 pM) until sibling embryos reached st. 20 and were then analyzed by RT-PCR (left panel). Animal caps pre-treated with Noggin mRNA (100 pg) and either control MO or Cdc25B-MO (36 ng) were cultured and analyzed by RT-PCR (right panel). (F) Embryos pre-injected with control MO or FoxM1-MO (36 ng) together with or without Cdc25B mRNA (200 pg) at the one-cell stage were cultured until st. 32 and photographed (left panels). For RT-PCR analysis (right panel), embryos were collected at st. 15. Image published in: Ueno H et al. (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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