Xenbase Image ID: 148119

FIGURE 9. GRAF1 is highly expressed during somitogenesis. A, RT-PCR analysis for x.GRAF1 and histone H4 (H4; loading control) was performed using RNA isolated from embryos at the indicated stages. B and C, whole-mount in situ hybridization of stage 29 Xenopus embryo using an antisense probe specific for x.GRAF1: h, heart; b, brain; s, somites; nt, neural tube; drg, dorsal root ganglia. B, lateral view. C, top, transverse; bottom; cross-section through mid-somite region (somite borders indicated with brackets). D, Western blot analysis of GRAF1 and ERK (loading control) expression at the indicated developmental stages. E, laser scanning confocal images of whole-mount GRAF1 (red), tropomyosin (green), and ToPro3 (blue nuclei) stained somites from wild type stage 37 embryo. IHC, immunohistochemistry. Image published in: Doherty JT et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue arhgap26.L graf, graf1, ophn1l, ophn1l1 X. laevis Throughout NF stage 29 and 30 somite lens anterior ventral blood island heart spinal ganglion neural tube pharyngeal arch central nervous system brain spinal cord eye arhgap26.L graf, graf1, ophn1l, ophn1l1 X. laevis Throughout NF stage 29 and 30 somite tnnt2.L CT3, LOC446916 X. laevis Throughout NF stage 37 and 38 muscle somite Image source: Published Larger Image Printer Friendly View

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