Xenbase Image ID: 148123

Supplemental Figure 4. Characterization of GRAF-specific antibody. A. COS cells were transfected with constructs containing flag-tagged human or Xenopus GRAF cDNAs. Lysates were immunoblotted using either anti-Flag or the GRAF-specific Ab. Both antibodies recognize a single protein species at approximately 110kDa. B. Extracts obtained from stage 32 Xenopus embryo or adult skeletal muscle (sk.m.) or heart were immunoblotted with GRAF-specific Ab. Lysate from COS cells transfected with Xenopus F-GRAF (XFGRAF) is shown as a positive control. Image published in: Doherty JT et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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