Xenbase Image ID: 148127

Supplemental Figure 8. GRAF depletion does not affect somite rotation or myoseptal alignment but leads to progressive loss of segmental integrity. A,B) Laser scanning confocal microscopy and digital deconvolution of whole-mount stage 25 Con Mo- and GRAF Mo-injected embryos stained with designated antibodies. Note appropriate alignment of nuclei at the mid-point of organized myoseptal boundaries (demarcated by LAM, -dystroglycan, and paxillin). Panel B shows example of mild phenotype observed in 3/25 Stage 25 GRAF morphants. C. Stage 37 Con Mo- and GRAF Mo-injected embryos co-stained with - dystroglycan (green) and TOPRO (nuclei, red) reveals progressive disruption of myoseptal boundaries in late stage GRAF morphants. Image published in: Doherty JT et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue dag1.L alpha-dystroglycan, dystroglycan X. laevis Throughout NF stage 25 somite pxn.S paxillin X. laevis Throughout NF stage 25 intersomitic epithelium intersomitic region dag1.L alpha-dystroglycan, dystroglycan X. laevis Throughout NF stage 37 and 38 intersomitic region intersomitic epithelium Image source: Published Larger Image Printer Friendly View

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