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Fig. 2.
Expression patterns of gata2, bcar3 and bcar1 during Xenopus development (A) Northern analysis showing bcar3 RNA transcripts. odc was used as a loading control. All lanes are from the same blot, aligned following removal of intervening lanes (following the 2nd and 3rd lanes) using Photoshop. N/F: Nieuwkoop and Faber stages. (B-II) WMISH analysis of expression of gata2 (B–D and M–Q), bcar3 (E–H, R–V and BB–EE) and bcar1 (I–L, W–AA and FF–II) during development. For each stage, the same embryo is shown in animal, vegetal, lateral, dorsal or ventral views. The embryos in panels CC and GG were mounted in Murray's clear to visualize internal structures. Brown is pigment. All other embryos were photographed in PBS to visualize superficial structures. (DD, EE, HH and II) Transverse sections of previously stained st. 34 embryo at the level shown in panels CC and GG respectively. Np: neural plate, n: notochord, anf: anterior neural folds, b: brain, sm: somitic mesoderm, e: eye, cg: cement gland, g: cranial ganglia, sc: spinal cord, fm: fin mesenchyme, tb: tail bud, ma: abdominal muscle anlagen, p: pronephros, c: cloaca, ba: branchial arches, vbi: ventral blood island.
