Xenbase Image ID: 148341

Figure 1. Molecular Cloning and Expression of XSH-PTP2 (A) Deduced amino acid sequence for XSH-PTP2. SH2 domains are indicated by solid lines and the PTP domain by italics. (13) Comparison of the structure of XSH-PTP2 to HSH-PTP2 and csw, indicating a high degree of conservation. Percent identities are indicated. XSH-PTP2 and HSH-PTP2 lack an insert in the phosphatase domain. Two potential tyrosine phosphorylation sites are conserved from XSH-PTP2 to HSH-PTP2; one is also found in csw. All three homologs retain a proline-rich sequence within their C-termini. (C) Northern blot of SH-PTP2 expression in early Xenopus development. Numbers across the top indicate developmental stage. XSHPTP2 is expressed as an approximately 3 kb transcript, present in oocytes (M) and fertilized eggs (stage 1) through tadpole (stage 38). The blot was reprobed with Xenopus fibronectin to control for RNA integrity. Fibronectin RNA levels increase in the later stages of normal embryogenesis. The apparent decrease in XSH-PTP2 RNA expression in mid-embryonic stages is not observed reproducibly. (D) Maternal expression of XSH-PTP2 protein. Recombinant HSHPTP2 (50 and 100 ng) in total cell lysates from baculovirus-infected Sf9 cells (lanes A and B, respectively) and soluble protein from the equivalent of one-half (1/2) or one oocyte (lanes C and D, respectively) were detected by immunoblotting with anti-PTPID/SH-PTP2 monoclonal antibody. Image published in: Tang TL et al. (1995) Copyright © 1995. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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