XB-IMG-148343
Xenbase Image ID: 148343
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Figure 3. AP RNA Injection Results in Embryos with Severe Tail Truncations
Four- to eight-cell embryos were injected in the dorsal marginal zone
with wild-type XSH-PTP2 or AP RNA and allowed to develop•
(A-D) Morphology of injected embryos. Embryos were scored for developmental
abnormalities at stage 18 (A and B) and stage 44 (C and
D): embryos injected with XSH-PTP2 RNA (A and C); embryos injected
with AP RNA (B and D). The phenotype of AP-injected embryos at
stage 18 reflects failure of blastopore closure at the end of gastrulation.
At stage 44, the extreme tail truncation of AP-injected embryos is
evident•
(E and F) Histological analysis. A transverse section at the back of
the head of a normal, XSH-PTP2 RNA-injected tadpole is shown in
(E). Brain (b), somites (s), and notochord (nc) are indicated. The scale
bar in (E) represents 100 I~m, For comparison, in a more posterior
section, a AP RNA-injected embryo is shown (F). Split notochord and
neural tube (nt) are evident; somites are not duplicated•
(G) Dose response of AP RNA injection. Increasing amounts of AP
RNA were injected into 4- to 8-cell embryos, which were allowed to
develop to stage 18 and scored for tail truncation.
(H) Inhibition of Xbra expression by injection of AP RNA. Northern
blot of RNA from stage 11 embryos, injected in the marginal zone of
all four blastomeres at the 4-cell stage with water control (C), SH-PTP2
PTP domain mutant RNA (AP), wild-type XSH-PTP2 RNA (WT), dominant
negative FGFR RNA (FRD), or wild-type FGFR RNA (FR), andprobed with the indicated early mesodermal markers. Injection of either
AP or FRD inhibits Xbra expression. The same blot rehybridized
with a fibronectin probe (FN) serves as a control for RNA loading and
integrity. Image published in: Tang TL et al. (1995) Copyright © 1995. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |