XB-IMG-148413
Xenbase Image ID: 148413
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Fig. 1. Flow diagram of the protocols for animal rearing, cell isolation, RNA preparation and microarray hybridization. At stage 46 or 48, tadpoles were electroporated with a GFP-expression construct and placed in one of three visual experience conditions: normal 12 h light:12 h dark conditions; visual deprivation (vd), or enhanced visual experience. These rearing conditions produced 5 cell groups: active NPCs (aNPCs), Mature Neurons, Immature Neurons, Active NPCs isolated from visually-deprived tadpoles (aNPCvd), and quiescent NPCs (qNPCs). See text for details. GFP+ cells were harvested from dissociated midbrains and RNA was isolated and prepared for microarrays. The bottom panel shows which samples were compared by microarray analysis to identify differentially expressed genes that might be involved in cell proliferation and neurogenesis. Image published in: Bestman JE et al. (2015) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View |