XB-IMG-148424
Xenbase Image ID: 148424
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Fig. 2. Effect of Nodal signal on gene expression of pou5f3.2.
Synthesized RNA of Xnr2 was injected into both blastomeres of
2-cell stage embryos. Animal caps were isolated from the Xnr2-
injected embryos at St.8, and were reared until St.10.5 with or
without cycloheximide (CHX). Total RNA was extracted from the
animal caps and gene expression of pou5f3.2 was examined by
RT-PCR using reduced number of PCR cycles to detect an effect
of Xnr2. Gene expression of Xbra1 was examined as a positive
control for Nodal signaling. Histone H4 is an internal control. -RT:
without reverse transcriptase reaction. Image published in: Nishitani E et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |