Xenbase Image ID: 148428

Fig. 6. Gene expression of p27Xic1 in pou5f3.2-injected embryos. (A) Whole-mount in situ hybridization showing p27Xic1 expression at St. 10.5. The embryo was injected with 800 pg GFP RNA or pou5f3.2 RNA into the animal pole at 1-cell stage. Lateral view with the animal pole upside. (B) RT-PCR analysis of p27Xic1 expression in the pou5f3.2-injected embryo at St.10.5. Histone H4 is an internal control. -RT: without reverse transcriptase reaction. (C) RT-PCR analysis of p27Xic1 expression in the pou5f3.2MO-injected embryo at St. 10.5. Histone H4 is an internal control. -RT: without reverse transcriptase reaction. Image published in: Nishitani E et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue cdknx.L p27Xic-1, p27XIC1, p28, Xic-1, Xic1 X. laevis Throughout NF stage 10.5 animal hemisphere Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + pou3f2 + NF10.5 (in situ hybridization) fig.6.a Expression Phenotype decreased amount cdknx.S expression in animal hemisphere Larger Image Printer Friendly View

Return to previous page