Xenbase Image ID: 148523

Fig. 3. (A and B) show immunoblots of Triton-insoluble proteins from 10 defolliculated oocytes, either uninjected (-) or injected with 5ng of synthetic vimentin mRNA 20 hours prior to preparation of the extract (+). A is blotted with affinity-purified CK7 antibody (2^g/ml), and B with affinity-purified Z9 (5/^g/ml). Arrows show MT 55, 56 and 57,000 proteins. Overexpressed vimentin is detected by Z9 (B; lane 1), but not by CK7 (A; lane 1). C shows Triton extracts of 5 stage 5, 10, 17, 23 embryos, and 2 stage 38 embryos blotted with CK7 (2ug/ml) Image published in: Torpey NP et al. (1992) Copyright © 1992. Image reproduced with permission of the Publisher, The Company of Biologists Ltd. Larger Image Printer Friendly View

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