Xenbase Image ID: 148643

Figure 7. Slit2 is required for MCC insertion into the overlying epithelium. (A) A control embryo injected with membrane-RFP and α-tubulin > membrane-GFP to label MCCs. Injected embryos were then fixed and stained with RFP, GFP, and α-acetylated tubulin. (B) Slit2 morpholino was injected with membrane-RFP and α-tubulin > membrane-GFP. Note that MCCs fail to insert into the mucociliary epithelium following Slit2 knockdown (arrows). In addition, MCCs (arrowheads) with no Slit2 morpholino insert into the superficial layer containing Slit2 morpholino. These data indicate Slit2 controls MCCs in a cell-autonomous fashion. Image published in: Chung MI et al. (2014) Copyright © 2013, Chung et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 27 ciliated cell Image source: Published Larger Image Printer Friendly View

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