XB-IMG-148676
Xenbase Image ID: 148676
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Figure 2. Inhibition of mesoderm, endoderm, and epidermis formation
in animal pole explants. A, Inhibition of mesoderm markers induced by
FGF examined at stage 10.5. WE is the positive control for RT-PCR,
using the cDNA from whole embryos at the equivalent stage. RNA
injected per embryo: 0.1 ng for E1A, RG, and DL; 3 ng for p300N. bFGF
concentration is 50 ng/ml. B, Inhibition of mesoderm and endoderm
markers induced by activin examined at stage 10.5. mix1 is a homeobox
gene expressed in both mesoderm and endoderm. Endodermin induction
by activin was examined at stage 35. The RNA amount that was injected
is the same as in A. Activin concentration: 5 ng/ml (for mesoderm
induction) and 50 ng/ml (for endoderm induction). C, Inhibition of early
BMP-4 target genes induced by an activated ALK2, a BMP-4 receptor.
RNA injected per embryo: 0.05 ng for E1A, RG, and DL; 3 ng for p300N;
0.2 ng for ALK2. D, A lack of inhibition of Xwnt-8 or b-catenin signaling.
RNA injected per embryo is the same as in A, except 10 pg for Xwnt-8 and
0.1 ng for b-catenin. E, E1A or p300N inhibition of mesodermal and
endodermal gene expression was rescued by p300(dl10), which has wildtype
p300 function but is refractory to E1A inhibition (Eckner et al., 1994;
Lee et al., 1996). RNA injected per embryo is the same as in A, except 1
ng for p300(dl10). Image published in: Kato Y and He X (1999) Copyright © 1999. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |