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Xenbase Image ID: 148678

Figure 4. Rescue of early neural development in UV-ventralized embryos. RNA injected per embryo: 20 pg for E1A and RG, 3 ng for p300N, and 150 pg for chordin. In situ hybridization and RT-PCR were performed on embryos at stage 14. A, Rescue of zic-r1 expression by E1A and chordin, but not by RG2 or b-galactosidase (used as a lineage-tracing marker). Note that the expression of zic-r1 overlaps with X-gal staining in E1A or chordin (chd)-injected embryos. B, Rescue of neural markers examined by RT-PCR.

Image published in: Kato Y and He X (1999)

Copyright © 1999. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

zic1.SZic-r1, opl, odd-paired-like, Zic-related-1X.laevisThroughout NF stage 13neuroectoderm

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