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Figure 2—figure supplement 4. Validation of Rfx2-dependent genes.(A) Overview of the animal cap assay. (B) RT-PCR results. Ctl: control animal caps. Rfx2 MO: Rfx2 morpholino-injected animal caps. α-tubulin expression was not changed. ef1α served as a loading control. (C) ccdc63, ccdc104, dnal1, ribc2, ropn1l, tekt3, and ttc29 were expressed in MCCs and their expressions were Rfx2-dependent.DOI:
http://dx.doi.org/10.7554/eLife.01439.010 Larger Image Printer Friendly View |