Xenbase Image ID: 148698

Figure 4—figure supplement 2. Nme5 is required for ciliary motility.(A and B) A MCC of a stage 27 embryo injected with Nme5-GFP and membrane-RFP. (C) A MCC of a stage 27 control embryo injected with membrane-GFP. (D) A MCC of a stage 27 embryo injected with Nme5 morpholino. Ribc2 is not essential for cilia assembly. Tracking of latex beads moving across the epidermis of the control embryo (E) and the Ribc2 morphant (F). An arrow represents the moving distance per time frame. The relative average flow rate is shown in (G). While the average flow rate of control is normalized to 1 ± 0.036 (mean ±SEM), it is significantly reduced to 0.24 ± 0.011 in Nme5 morphants. Control: n = 42, 3 embryos. Ribc2MO: n = 88, 3 embryos. ***p < 0.0001 Mann–Whitney test. Scale bar: 10 µm.DOI: http://dx.doi.org/10.7554/eLife.01439.016 Larger Image Printer Friendly View

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