XB-IMG-148743
Xenbase Image ID: 148743
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Fig. 2. Xenopus syntabulin is a vegetally localized maternal mRNA. (A) Whole-mount in situ hybridization on stage VI oocytes using an antisense probe showing that maternal syntabulin (sybu) transcripts are localized at the center of the vegetal pole. The inset shows the sense probe negative control. (B) In situ hybridization on albino stage VI oocytes showing that syntabulin transcripts form a granular pattern in the vegetal cortex; note arrow in the hemisection shown in the inset. The germinal vesicle is indicated by a dotted line in inset. (C) In situ hybridization of sybu at different stages of oogenesis (I–V) showing that maternal transcripts localize early (stage I, arrow) in the mitochondrial cloud, and in the cortex of the vegetal pole at later stages. Dotted line in stage I oocyte marks the nucleus. An=animal pole; Veg=vegetal pole; GV=germinal vesicle. Image published in: Colozza G and De Robertis EM (2014) Copyright © 2014. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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