XB-IMG-148768
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Fig. 3.
Effects of eif6 downregulation. (a), (b) Serial cross-sections of stage 28 eif6-direct morpholino and GFP-injected embryo were immunostained with anti-GFP, with anti-eif6 antibodies and with TUNEL assay. The lower level of eif6 immunostaining (b) is present in the injected side (i.s.) marked by GFP (a). An increase of apoptotic nuclei is evident in the injected side (c). (d) Western blot with anti-eif6 antibody indicates that the level of protein in eif6 morpholino-injected embryos was lower than that in mispaired morpholino-injected embryos. Three sets of injected embryos were used in the blot. Normalisation was performed with anti-a-tubulin antibody. (e)–(h) Experimental and control side of the same embryo injected with eif6-direct morpholino (e), (f) or control mispaired morpholino (g), (h) in one blastomere at the two-cell stage and harvested at stage 35. No eye difference was evident between the injected side (e), (g) marked by GFP fluorescence (inset) and the un-injected side (f), (h). (i)–(n) In situ hybridisation of morpholino (i, k, m) or mispaired morpholino (j, l, n) injected embryos at the neurula stage. The expression of anterior markers rax (i), (j), pax6 (k), (l) and otx2 (m), (n) was not affected by depletion of eif6.
Image published in: De Marco N et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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