XB-IMG-148770
Xenbase Image ID: 148770
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Fig. 5.
Effects of mutant S235A overexpression. Embryos injected with eif6 mutant S235A (300 pg) and GFP (200 pg) or GFP alone (400 pg) in one blastomere of the two-cell stage were harvested at stage 35 (a)–(h) or at the neurula stage (i)–(n). (a) In the Western blots using anti-β-catenin antibody, eif6 or GFP-injected embryos and two sets of S235A-injected embryos were used. A decrease in β-catenin level was found only in eif6 overexpressors. Normalisation was performed with the anti-tubulin antibody. (b) RT-PCR analysis showed that β-catenin mRNA level was unaffected in eif6 and S235A overexpressors compared with GFP-injected embryos. Normalisation was performed using histh4 primers. (c)–(h) Micrographs of the two sides (c and d; e and f; g and h) of the same stage 35 embryo are given. S235A overexpressors show a reduction in eye size (arrowheads) of the injected side (c), (e), marked by GFP fluorescence (inset), compared to the uninjected side (respectively d, f). (g), (h) Injection of GFP alone (fluorescence in inset of Figure e) did not affect eye development. (i)–(n) Whole mount in situ hybridisations were performed for rax (i), (j), pax6 (k), (l) and otx2 (m), (n) on GFP (i, k, m) or S235A (j, l, n) overexpressing embryos at neurula stage. The expression of the eye marker rax (j), pax6 (l) and otx2 (n) was inhibited in the eye field (arrows) of the S235A-injected side. Image published in: De Marco N et al. (2011) Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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