Xenbase Image ID: 148771

Fig. 1. Increase in ERK MAPK phosphorylation following TAK1 knockdown. Embryos were injected with 20 ng of either the TAK1 MO (TAK MO) or the 5-base mispair (mis MO). Tissues were isolated when embryos reached early gastrula (10–10.25). (A) Activated (diphospho; dp-MAPK) and total ERK MAPK (MAPK) from isolated ectoderm. (B) Activated (diphospho; dp-MAPK), and total ERK MAPK (MAPK) from isolated mesoderm. This mesoderm consists of the lower (i.e., vegetalward) marginal zone minus the superficial layer, around the entire circumference of the embryo. (20 tissue isolates/sample; N=3 independent experiments). Image published in: Liu C et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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