Xenbase Image ID: 148774

Fig. 4. Reduction in Nuclear Smad1 in TAK1 morphants. Embryos were injected with 20 ng of either the TAK1 MO or the mispair MO and lysed at st. 10.25. (A) Nuclear and cytoplasmic fractions were immunoblotted and probed with antibodies directed against Smad1, diphospho-ERK MAPK (dp-MAPK), α-tubulin, and lamin B2. (B) Nuclear–cytoplasmic distribution of Smad1 in TAK1 morphant and control embryos. (C=Cytoplasmic, N=Nuclear). ***, p<0.0001. (C) Nuclear–cytoplasmic distribution of diphospho-ERK MAPK. Bars represent mean±S.E.M. N=3 independent experiments. Image published in: Liu C et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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