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Fig. 8. Effects of TAK1 knockdown on dorsal–ventral pattern. Embryos were injected in both dorsal blastomeres at the 4-cell stage with 20 ng of either the TAK1 MO (TAK MO) or the 5-base mispair (Mis MO); embryos were fixed at early gastrula stages (10+–10.25) and used for in situ hybridization. Dorsal-specific probes include not1 (A, B, A′, B′), otx2 (C, D), and shh (E, F). Ventrolateral probes include Xvent2 (G, H), and Xvent1 (I, J). Expression of each gene was evaluated in samples from 4 independent experiments, prepared in 2 rounds of in situ hybridization. Each set of images (for a given probe) was taken from the same round of in situ hybridization; thus, mispair and TAK1 MO embryos were processed identically, and in situs were developed for the same period of time. Images show expression patterns characteristic of at least 2/3 of embryos (TAK vs. mispair MO). Image published in: Liu C et al. (2012) Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue not.S flh, floating head, not-a, not-b, not1, noto, Xnot, Xnot-2, Xnot1, Xnot2, Znot X. laevis Sometime during NF stage 10 to NF stage 10.25 marginal zone dorsal marginal zone shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Sometime during NF stage 10 to NF stage 10.25 marginal zone dorsal marginal zone upper blastopore lip involuting marginal zone otx2.S otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 X. laevis Sometime during NF stage 10 to NF stage 10.25 marginal zone dorsal marginal zone upper blastopore lip involuting marginal zone ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Sometime during NF stage 10 to NF stage 10.25 marginal zone blastopore lip ventro-lateral marginal zone involuting marginal zone ventx1.2.S vent-1, vent1, Xvent-1, Xvent1 X. laevis Sometime during NF stage 10 to NF stage 10.25 marginal zone ventro-lateral marginal zone blastopore lip involuting marginal zone Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + map3k7 MO + NF10-10.25 (in situ hybridization) fig.8.b, b^1 Expression Phenotype mislocalised not.S expression in marginal zone increased amount not.S expression in marginal zone Xla Wt + map3k7 MO + NF10-10.25 (in situ hybridization) fig.8.d Expression Phenotype increased amount otx2.S expression in dorsal marginal zone mislocalised otx2.S expression in dorsal marginal zone Xla Wt + map3k7 MO + NF10-10.25 (in situ hybridization) fig.8.f Expression Phenotype mislocalised shh.L expression in dorsal marginal zone increased amount shh.L expression in dorsal marginal zone Xla Wt + map3k7 MO + NF10-10.25 (in situ hybridization) fig.8.h Expression Phenotype mislocalised ventx2.2.L expression in marginal zone increased amount ventx2.2.L expression in marginal zone Xla Wt + map3k7 MO + NF10-10.25 (in situ hybridization) fig.8.j Expression Phenotype increased amount ventx1.2.S expression in marginal zone mislocalised ventx1.2.S expression in marginal zone Larger Image Printer Friendly View

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