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Fig. 9. Effects of TAK1 MO on the spatial extent of FGF/MAPK signaling. All animal caps (AC) were held under a coverslip for 30 min, fixed, and stained with the anti-diphospho-MAPK antibody. (A) AC without a bead. (B) AC with bead soaked in PBS. (C–E), AC exposed to beads soaked in FGF. Lines indicate extent of diphospho-MAPK immunoreactivity. The holes indicate bead sites. (C) AC injected with β-galactosidase mRNA. (D) AC injected with noggin mRNA. (E) AC injected with TAK1 MO. For each preparation, N=≥6 explants in each of ≥3 independent experiments for each type of sample.

Image published in: Liu C et al. (2012)

Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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