Xenbase Image ID: 148823

Fig. 2 Embryonic expression of ube2d3.2. (now called ube2d4) (A) A polyclonal antibody raised to amino acids 1–147 of ube2d3.2 was used to probe control embryos (uninjected) and embryos expressing FLAG-tagged ube2d3.2(-FLAG). (B) Equal quantities of total embryonic protein from the indicated stages were loaded onto an SDS-PAGE gel and probed with the polyclonal antibody raised against ube2d3.2, upper panel. A duplicate membrane was probed with pre-immune serum from the rabbit used to produce the specific ube2d3.2 antibody, middle panel (both the upper and middle panels were exposed for the same time). Tubulin was used as a loading control lower panel. ( C) Expression pattern of ube2d3.2 mRNA at stages 28 and 35. Panels show lateral views with anterior to left and the right panel is a magnification of the middle panel. AS, antisense probe. The somites (s), eye (e), otic vesicle (o), branchial arches (ba), and pronephric primordium (p) are indicated. Image published in: Jean S and Moss T (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue ube2d4.L HBUCE1, ube2d3.2 X. laevis Throughout NF stage 28 intermediate mesoderm otic vesicle somite presomitic mesoderm pronephric mesenchyme optic vesicle ube2d4.L HBUCE1, ube2d3.2 X. laevis Throughout NF stage 35 and 36 pronephric kidney otic vesicle somite presomitic mesoderm brain tail pharyngeal region Image source: Published Larger Image Printer Friendly View

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