Xenbase Image ID: 148901

Figure 1. Imaging of calcium dynamics. (a) Emission filters and dichroic mirrors used here were designed to reflect laser light efficiently at 488 nm but transmit 90% of light at 505 nm, collecting a large fraction of the emission spectrum of calcium green. (b) The experimental design for the observation of calcium dynamics in the Xenopus DMZ. Embryos were injected with calcium green dextran at the 4-cell stage (1) and cultured to early gastrula stages. Open-face DMZ explants were then prepared (2) with deep cells apposed to the coverglass (3). (c) The confocal system used allows effective, long-term confocal time-lapse of living cells. The left panel shows a field of cells from a calcium green dextran-labeled DMZ explant after one 8 s scan; the right panel shows the same field of cells after 254 additional 8 s scans over 85 min. The mean fluorescent intensity was reduced by only 6% Image published in: Wallingford JB et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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