Xenbase Image ID: 148904

Figure 4. and Movies 5 and 6 Calcium waves and calcium flashes. (a) A high-magnification view of cells involved in the wave described in Figure 2. During the 4 minutes preceding the wave, very little movement is observed (t = :00–4:00). The yellow outline at t = 4:40 indicates the position of two cells just prior to the calcium wave. The calcium wave propagates from left to right across the field of cells between t = 5:00 and t = 5:40. As the wave moves across and begins to dissipate, cells move dramatically (compare initial position to cells at t = 7:20). As the calcium levels recover, the cells move dramatically in the opposite direction, beyond their original position (t = 8:00–12:00). The scale bar indicates 50 μm. (a′) The plot of δF/F0 for each of the areas shown in the colored boxes in the last panel of (a) shows that local changes in calcium levels reflects the pattern of calcium release and recovery in the overall wave (compare with Figure 2a′). (Movie 5) This movie shows the cells in Figure 4a. (b) A high-power view of cells involved in a calcium flash. Three cells dramatically increase calcium levels between t = :00 and t =:20. By t = 1:00, calcium levels are decreasing and return to baseline by t = 1:20. (b′) The plot of δF/F0 for each of the areas shown in the colored boxes in the last panel show that the rise in calcium levels does not propagate to other cells in the frame. (Movie 6) This movie shows the calcium flash in Figure 4b Image published in: Wallingford JB et al. (2001) Copyright © 2001. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page