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XB-IMG-148940

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Accumulation kinetics of pri-mir427 per allele in Clutch A rdRNA, see also Fig S7 A – pri-mir427 expression over first 12 hpf in rdRNA data and in situ hybridizations. pri-mir427 show ubiquitous expression matching RNA-seq temporal data. Examples of most abundant transcripts shown for comparison. Inset: first detection of pri-mir427 above detection limit at 2.0 hpf (8–16 cell transition). B – pri-mir427 abundance in kb per cell. Line marks Gaussian process median, error bar is 95% CI (Supplemental Experimental Procedures). Peak per cell abundance occurs after 11th division at 4.4 hpf. C – pri-mir427 accumulation rate in kb/min/allele with time. Line is median of differential of Gaussian Process, error bar gives 95% CI (Supplemental Experimental Procedures). Peak accumulation rate is achieved at 4.2 hpf just after 10th cell division. D – Organization of pri-mir427 locus on X. tropicalis Chromosome 3 (X. tropicalis v8 genome, Supplemental Experimental Procedures). Clusters of mir427 hairpins appear in tandem with a repeated sequence arranged symmetrically on opposite strands around a gap in the genome assembly.

Image published in: Owens ND et al. (2016)

Copyright © 2016. Image reproduced on Xenbase with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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