Xenbase Image ID: 148956

Fig. 2. Cardiac cell diversification is lost in dissociated and reaggregated animal cap explants but can be restored by blocking BMP signalling. (A) Restricted cardiac gene expression is lost in GATA4 injected explants dissociated and reaggregated. Inhibition of BMP signalling through dominant-negative BMP type I receptor (tBr) restores ventricular and proepicardial gene expression in GATA4 co-injected reaggregated explants. (B). Ventricular gene expression (myl3) is partially restored in explants also co-injected with GATA4 and Dkk-1 mRNA. (C) The second proepicardial marker, WT1, shows the same pattern of expression as Tbx18 in intact (Fig. 1K) and reaggregated animal cap explants expressing GATA4, GATA4 and Dkk-1 or GATA4 and tBr. AC, Animal Caps; G4, GATA4; R, dissociated and reaggregated animal caps. RT-PCR was performed at stage 40 according to control embryos. A representative experiment is shown out of 3 (A, B) or 2 (C). Image published in: Caporilli S and Latinkic BV (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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