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Fig. 3. Ventricular and proepicardial gene expression is restored in reaggregated animal cap explants expressing GATA4 upon transplantation into host embryos. (A–F) Dissociated and reaggregated GATA4 injected explants (CAG-GFP) were transplanted at stage 20 into host (CAG-GFP) at the same stage. GFP expression was recorded at stage 39. (C, F) lineage tracing (rhodamine dextran) of transplants in D and E. (G, H) Transplants were analysed for the expression of myl7 and myl3. 48% of transplants (n = 45) showed patterned gene expression with regions of overlapping expression between myl7 and myl3 corresponding to the ventricle and regions expressing myl7 alone. 30% of transplants expressed only myl7, and in 17% there was a complete overlap between myl7 and myl3. (G′, H′) Close-up of transplants shown in G and H. Black head arrow shows overlapping gene expression and white head arrow shows region of myl7 expression alone. (I, J) 65% of transplants (n = 30) express both Tbx18 and myl7. (I′,J′) Close-up of transplants shown in I and J. h, heart; ih, interhyoid muscle of the jaw; p, proepicardium; v, ventricle. The scale bar corresponds to 100 μm. Image published in: Caporilli S and Latinkic BV (2016) © 2016 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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