Xenbase Image ID: 148987

Figure S4. Sufficiency of human GMNC over-expression in MCC specification and induction of MCC genes in Xenopus embryos (A-C) Embryos injected with 80pg human GMNC RNA (B) displayed ectopic MCCs compared to uninjected embryos (A) at stage 29-30. Acetylated-tubulin (Ac; white). Scale bars: 10μm. Quantification was done by capturing three representative images in the mid-section of the embryos along the anterior-posterior axis, and taking the average of the number of multi-cilia bundles in each field of view (C). Error bars represent SD (n=3). P=0.0038 (**), based on unpaired t test. (D) Quantitative qPCRs were performed on uninjected or 80pg human GMNC RNA-injected (hGMNC) embryos. hGMNC overexpression induced foxj1 level by ~20-fold, and mcidas level by ~5-fold. Expression levels in the uninjected condition were arbitrarily assigned a value of 1. ornithine decarboxylase (odc) was used as an internal (loading) control. Error bars represent SEM from 2 independent experiments. Image published in: Zhou F et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 29 and 30 multiciliated cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + Hsa.gmnc + NF29/30-29 and 30 (immunohistochemistry) Fig. S4. B. C Expression Phenotype increased amount tuba4b.L expression in multiciliated cell Xla Wt + Hsa.gmnc + NF29/30-29 and 30 (RT-PCR) Fig. S4. D Expression Phenotype increased amount foxj1.L expression in embryo Larger Image Printer Friendly View

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