Xenbase Image ID: 148992

Sup. Fig 6. Pax7 splice MO phenotype. A splice-blocking morpholino was designed (see Supplemental Fig. 2). Injections in whole embryos were monitored for pax7 expression by semi-quantitative RT-PCR followed by phospho-imager analysis. This shows that the Pax7 splice MO decreases pax7 expression by 60% at the dose used here, at tailbud stage 22 (A). The morphology of the embryos (whole embryo injections) was similar to the one observed for Pax7MO (translation blocking MO) (B) with a slightly milder percent of severe embryos. Marker genes analyzed during neurulation or later on displayed similar defects as those observed with the Pax7MO (see Fig. 6). Image published in: Maczkowiak F et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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