Xenbase Image ID: 149039

Figure 4. E-Syt2 Interacts with Adaptin-2 to Modulate Receptor Endocytosis, ERK Activation, and Xbra Induction(A) E-Syt2 interacts with the endogenous AP-2 tetramer. HA-E-Syt2 and Myc-tagged FGFR1 (Myc-FGFR1) were coexpressed in HEK293T cells stimulated or not with bFGF. Endogenous AP-2 complexes were immunoprecipitated with a β2-Adaptin-specific antibody or a nonspecific control antibody (IgG Ctrl) and I.B.(B) Both E-Syt2 and FGFR1 interact with the AP-2 complex. β2-adaptin (FLAG-β2-Ad) was coexpressed with FGFR1 (Myc-FGFR1) and with increasing amounts of E-Syt2 (HA-E-Syt2) and complexes were immunoprecipitated with an anti-FLAG antibody (I.P. FLAG). The upper panels show immunoblots for the coprecipitated proteins and the lower panels show protein levels in the input protein lysate.(C) E-Syt2 gain of function also inhibits bFGF and activated FGFR1 (CA-FGFR1)-mediated, but not Activin-mediated Xbra induction in ACs.(D) Left panel, constitutively activated-ERK (CA-ERK) but not constitutively activated PI3K (PI3K-CAAX) expression rescues E-Syt2 inhibition of Xbra induction by CA-FGFR1 in ACs. Right panel, consistent with this PI3K-CAAX activation of Akt in ACs is not strongly affected by E-Syt2 gain of function.(E) E-Syt2 gain of function inhibits ERK activation by CA-FGFR1 (anti-phospho-Erk immunoblot) in ACs.See also Figure S4. Image published in: Jean S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page