Xenbase Image ID: 149042

Figure 7. The Interaction between FGFR1 and E-Syt2 Is Conserved in Human(A) Human E-Syt2b (HA-hE-Syt2b) was coexpressed with Xenopus or human FGFR1 (FLAG-xFGFR1 or -hFGFR1) in HEK293T cells, the receptors immunoprecipitated and proteins analyzed by immunoblotting.(B) Endogenous human E-Syt2 was immunoprecipitated from a whole-cell extract using xE-Syt2 antibody #2 and the precipitate immunoblotted for both hE-Syt2 (hE-Syt2#Pr0863-2932r, see Experimental Procedures) and hFGFR1 (Flg #121, Santa Cruz Biotechnology).(C) Human E-Syt2b interacts specifically with activated FGFR1. hE-Syt2b (HA-hE-Syt2b) was coexpressed with human FGFR1 (FLAG-hFGFR1) in HEK293T cells and receptor activation inhibited with SU5402. The receptor was then immunoprecipitated and proteins analyzed by immunoblotting.(D) Specific interaction between hE-Syt2b and activated hFGFR1 requires the N-terminal domain of hE-Syt2b. Full-length hE-Syt2b or the mutant FLAG-hδC2ABC containing aa 1–312 was coexpressed with hFGFR1 in HEK cells as in (C). Cells were then treated with either bFGF or with the inhibitor SU5402 before immunoprecipitation and analysis of FLAG-positive complexes.(E) Potential E-Syt2 functions in FGF signaling. Two possible modes of action are suggested; see text for more detail.See also Figure S7. Image published in: Jean S et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page