XB-IMG-149094
Xenbase Image ID: 149094
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Figure 1. A Shift in Glycolysis and Gluconeogenesis Drives Glycogen Accumulation in Oocytes(A) Periodic acid Schiff’s staining of glycogen in Drosophila follicles. Scale bar, 50 μm.(B) GC/MS measurements of select glycolytic intermediates and sugars from stage 10 and stage 14 follicles.(C) GC/MS measurements of TCA cycle intermediates from stage 10 and stage 14 follicles.(D) GC/MS measurements of the byproducts of purine breakdown from stage 10 and stage 14 follicles.(E) Model of both the glycolysis and TCA pathways. Highlighted in red are the compounds that increased at least 3-fold in stage 14 oocytes.(F) Colorimetric glycogen measurements of stage 14 oocytes from pepckGS/pepckGS mutant females.(G) Colorimetric glycogen measurements of stage 14 oocytes from DNaseIIlo/DNaseIIlo mutant females.(H) PAS staining of oocytes from DNaseIIlo/DNaseIIlo mutant females.Error bars represent SD. ∗p < 0.05; ∗∗p < 0.005. Image published in: Sieber MH et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |