Xenbase Image ID: 149095

Figure 2. Mitochondria Enter a Low-Energy State of Respiratory Quiescence to Promote Glycogen Storage in Late Oogenesis(A, C, and E) Ovaries from Oregon R females fixed and stained with ATP5a antibodies (red) and DAPI (blue).(B, D, and F) Ovaries from Oregon R females were stained with the mitochondrial membrane potential stain TMRE.(G) Stage-specific quantification of the % of TMRE-nurse cells from wild-type ovarioles.(H) Quantification of TMRE fluorescence from stage 8 and stage 14 follicle cells and nurse cells.(I) Complex I and III compound activity from early follicles, stage 14 oocytes, and 12- to 16-hr embryos.(J) Complex II and III compound activity from early follicles, stage 14 oocytes, and 12- to 16-hr embryos.(K) Complex II and III compound activity from early follicles, stage 14 oocytes, and 12- to 16-hr embryos.(L and M) GC/MS measurements of glycolytic (M) and TCA cycle (N) intermediates from stage 8 and stage 10 follicles.(N) qPCR quantification of relative nuclear and mitochondrial DNA content from stage 10 follicles and stage 14 oocytes.Error bars represent SD. ∗p < 0.05; ∗∗p < 0.005. Image published in: Sieber MH et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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