Xenbase Image ID: 149096

Figure 3. ETC Remodeling Drives Mitochondrial Respiratory Quiescence(A) Blue native PAGE of mitochondria from early-stage follicles and stage 14 oocytes.(B) Blue native PAGE of isolated mitochondria from stage 14 oocytes and 12- to 16-hr embryos.(C) Quantification of the ratio of complex I to complex IV from early follicles, stage 14 oocytes, and 12- to 16-hr embryos.(D) Quantification of the ratio of complex V to complex IV from early follicles, stage 14 oocytes, and 12-16 hr embryos.(E) Native western blot of ATP5a from early-stage follicles, stage 14 oocytes, and 12- to 16-hr embryos.(F) Whole-oocyte western blots of select mitochondrial proteins: ATP5a, NDUF3, COX IV, CYTOCHROME C, PORIN, and the loading controls ACTIN and TUBULIN from early-stage follicles, stage 14 oocytes, and 12- to 16-hr embryo samples.(G) Western blots of isolated mitochondria from early-stage follicles, stage 14 oocytes, and 12- to 16-hr embryo samples.(H) Electron microscope images of mitochondria from stage 8, stage 14 oocytes, and embryos.Error bars represent SD. ∗p < 0.05; ∗∗p < 0.005. Image published in: Sieber MH et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page