Xenbase Image ID: 149097

Figure 4. Insulin Regulates the Timing of Glycogen Accumulation and Mitochondrial Depolarization(A) Periodic acid Schiff’s staining (glycogen) of nos-GAL4/+ control and nos->InR-RNAi ovarioles.(B) TMRE staining and DIC images (below) of nos-GAL4/+ control and nos->InR-RNAi ovarioles.(C) Depicts the percentage of ovarioles that display premature glycogen accumulation from nos-GAL4/+ control, nos–Akt-RNA, nos–InR-RNA#1, and nos–InR-RNA#2 females.(D) Depicts the percentage of ovarioles that display a high level of mitochondrial membrane potential from nos-GAL4/+ control, nos–Akt-RNA, nos–InR-RNA#1, and nos–InR-RNA#2 females.(E) Normalized glycogen levels measured by colorimetric assay from nos-GAL4/+ control and nos–InR-DN stage 14 oocytes.(F) Normalized triglyceride levels measured by colorimetric assay from nos-GAL4/+ control and nos–InR-DN stage 14 oocytes.(G) p-AKT and AKT westerns from early-stage follicles, stage 14 oocytes, and 12- to 16-hr embryo samples.(H) Quantification of p-AKT/AKT ratios from early-stage follicles, stage 14 oocytes, and embryos.Error bars represent SD. ∗p < 0.05; ∗∗p < 0.005. Image published in: Sieber MH et al. (2016) Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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