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Figure 5. Akt Target Genes Are Required for Glycogen Storage in Stage 14 Oocytes(A) Glycogen levels were measured by colorimetric assay and normalized to total protein from the indicated genotypes. The results are displayed as a percentage of the wild-type control of each experiment.(B) Colorimetric triglyceride measurements of stage 14 oocytes from foxo21/foxo25 mutant females.(C and D) Glycogen (C) and triglyceride (D) levels were measured by colorimetric assay and normalized to total protein from nos-GAL4/+, UAS-gsk3-RNAi/+, and nos–gsk3-RNAi stage 14 oocytes.(E) Percent hatching of embryos from nos-GAL4/+, UAS-gsk3-RNAi/+, and nos–gsk3-RNAi stage 14 females.Error bars represent 1× SD. ∗p < 0.05; ∗∗p < 0.005.

Image published in: Sieber MH et al. (2016)

Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.

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