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Figure 6. GSK3 Promotes Mitochondria Respiratory Quiescence(A) TMRE staining and DIC images of nos-GAL4/+ control and nos–GSK3-RNAi stage 10B follicles.(B) Quantification the percentage of stage 10B follicles that exhibit high nurse cell mitochondrial membrane potential in control and gsk3-RNAI follicles.(C) Mitochondrial membrane potential staining (TMRE) from control fed, control starved, and gsk3-RNAi starved ovarioles.(D) Blue native PAGE of mitochondria from nos-GAL4/+ and nos–gsk3-RNAi stage 14 oocytes and dissected flight muscle (FM).(E) Quantification of the ratio of complex I to complex IV from nos-GAL4/+ and nos–gsk3-RNAi stage 14 oocytes.(F) Venn diagram of processes involving proteins found to be decreased in mitochondria from stage 14 oocytes. Each process is shown proportional to the number of affected proteins.(G) Venn diagram of processes involving proteins found to be stabilized in mitochondria from stage 14 GSK3-RNAi oocytes presented as in F.Error bars represent SD. ∗p < 0.05; ∗∗p < 0.005.

Image published in: Sieber MH et al. (2016)

Copyright © 2016. Image reproduced with permission of the Publisher, Elsevier B. V.

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