Xenbase Image ID: 149158

Figure 2 Multistep Lesion Bypass of an Interstrand Crosslink (A) Structure of the replicated AflIII fragment that includes the ICL. S, primer used to generate the sequencing ladder shown in (B). (B) Mapping of nascent strands during replication of pICLNm or pCtr (final concentration 1.2 ng/μl). At the indicated times after NPE addition, reaction products were digested with AflIII and analyzed on a sequencing gel alongside a sequencing ladder derived from extension of primer S on pCtr (see A). Numbers to the left indicate the sizes of the sequencing products. Leading and lagging strands for the rightward and leftward forks are indicated. Square brackets show the positions of leading strands after the initial pausing, whereas red and green arrowheads show their location after advancing toward the ICL. Open arrowhead, extension product. (C) Enlarged and darker exposure of the bottom part of the autoradiogram shown in (B). The most prominent species observed at 45 min are indicated on the right, with the predicted last nucleotide denoted by a single letter. The exact sizes of species T147-A150 were confirmed in Figure S9. (D) Cartoon-form depiction of the results in (C). Image published in: Räschle M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page