Xenbase Image ID: 149163

Figure 7 Model for ICL Repair in Xenopus Egg Extracts When DNA containing an ICL (A) undergoes DNA replication, the leading strands of two converging replication forks initially stall 20–40 nt from the lesion (B). One leading strand (indicated in red) is then extended to within 1 nt of the ICL, a step which may require prior replisome remodeling (C). Subsequently, the two sister chromatids are uncoupled via dual incisions (yellow scissors) on either side of the ICL, possibly by XPF and/or Mus81 (D). Next, a translesion DNA polymerase (possibly Rev1) inserts a nucleotide across from the adducted base (E), after which DNA polymerase ζ extends the nascent strand beyond the ICL (F). Finally, two fully repaired DNA duplexes are generated through the action of nucleotide excision repair (NER) on the top duplex and homologous recombination (HR) on the bottom duplex (G). Image published in: Räschle M et al. (2008) Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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