Xenbase Image ID: 149272

Fig. 1 (A) Fluorescence reporting from the vegetal pole. (A1) shows an embryo expressing GFP from mRNA injected bilaterally into the vegetal pole at the two-cell stage. There is little fluorescence in the vegetal pole itself, apart from yellow autofluorescence. (A2) strong vegetal fluorescence after injecting fluorescein-lysine-dextran. (A3) shows fluorescence from GFP is easily detected in the vegetal pole of a GFP mRNA-injected oocyte. (B) Primer extension analysis of transcription initiation from the endogenous Endodermin gene. The Endodermin promoter is diagrammed, marking the two TATA boxes at 65 and 285 bp, as well as the position of exon 1 and the primer used, 74 bp downstream of the ATG (green arrow). Below is an autoradiograph of the primer extension products of Endodermin, as well as Xsox17a as a control. On the left are dideoxy sequencing reactions, using the primer extension primers. The primer positions on the extension reactions are marked with white arrows and the predominant product with black arrows. In the case of Endodermin there is no single long extension band. The primer used is 50-AGGATGAGGAGGAGGTGCAG ACT-30. Image published in: Ahmed N et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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