XB-IMG-149275
Xenbase Image ID: 149275
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Fig. 4 Xsox17 can induce expression
of the E2 reporter construct. The
GFP in the E1 or E2/basal 169 bp
promoter fusions used for transgenesis
was replaced with firefly luciferase,
and 20 pg of these constructs
was injected bilaterally into the animal
pole, with or without 200 pg of
Xsox17b or Xsox17b::EnR mRNA,
or into the vegetal pole, with or
without Xsox17b::EnR. The basal
169/luciferase reporter is also
shown. Whole embryos were harvested
at early gastrula stages (10.5–
11) and groups of five embryos were
assayed in triplicate for firefly luciferase
activity. (A) Both E1 and E2
reporter constructs are induced
vegetally, but the basal promoter
is not. (B) In contrast, only E2 was
induced by Xsox17 in animal caps,
and it was repressed by Xsox17-
b::EnR. (C) Xsox17b::EnR repressed
E2 reporter expression by
80% in both the animal and vegetal
halves. E1 reporter expression
was also down-regulated by 40%
and 50% in the animal or vegetal
halves, respectively. Image published in: Ahmed N et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |