Xenbase Image ID: 149276

Fig. 5 A minimal promoter fragment E2:112, derived from E2, drives early expression in the endoderm of early Xenopus gastrulae. Deletion of site A from E2 does not affect endoderm expression (1), nor is this Sox site sufficient for endoderm expression (2). A GATA site and flanking sequence is also insufficient for endoderm expression (3). A reporter construct containing sites B and C drives endoderm expression (4), but a derivative that lacked site B did not (5). A reporter construct containing sequence upstream of site C, but which did not include site C itself, was also insufficient for endoderm expression (6). Endoderm expression was restored by addition of site B and upstream flanking sequence (7). A shorter version of this construct (E2:112) can also drive endoderm expression (8). The constructs are: (1) 2698/2326; (2) 2733/2698; (3) 2519/2326; (4) 2698/2508; (5) 2609/2508; (6) 2636/2550; (7) 2698/ 2550; (8) 2698/2586. Image published in: Ahmed N et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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