Xenbase Image ID: 149277

Fig. 6 (1) Site B is necessary but not sufficient for endoderm expression of E2:112. In each case, the constructs are shown on the left and GFP expression by fluorescence or in situ is shown on the right. (A, B) Endodermal expression of E2:112. (C–F) Reporter constructs containing site B, with either a 2 or 10 bp flanking sequence on either side, were not sufficient for endoderm expression. (G, H) Deletion of site B from E2:112 abolished reporter expression in the endoderm. (I–L) Endodermal expression was not restored in reporter constructs containing site B together with upstream or downstream sequences (I–L). (2) The reporter construct E2:112 is vegetally induced, but only if the B Sox site is present. Without this site, Xsox17b represses luciferase expression, but the B Sox site relieves this. The constructs are indicated. Image published in: Ahmed N et al. (2004) Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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