Xenbase Image ID: 149503

Fig. 4. Astrocytes are the primary phagocytes at metamorphosis. (A) Microglia, labeled by IB4-lectin-FITC (IB4 lectin, green), found in the ON parenchyma and laminin-immunolabeled ON sheath (red) at stages 58 and 66. Nuclei are labeled with DAPI (blue). (Scale bar: 50 μm.) IB4 signal per unit area in ON parenchyma (B) and ON sheath (C) is shown. (D) Astrocytes, immunolabeled with Blbp (green), express mRNAs (red) for phagocytic genes lrp1, mfge8, rac1, and many others (Fig. S4C), shown at stage 62. (Scale bar: 50 μm.) In B and C, the IB4 signal per unit area in individual ONs (n = 5) is shown with the mean ± SD. *P < 0.05 by Tukey’s test. (E) Myelin/axoplasm ratio per axon averaged per ON at stage 66. (F) Percentage of axon area that represents focal myelin detachments per axon at stage 66 (n = 12 control and n = 8 TR∆C in E and F). Values derive from 5,418, 3,014, and 3,450 axons in the control, Blbp:TR∆C, and Mbp:TR∆C groups, respectively. Image published in: Mills EA et al. (2015) Copyright © 2015. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue mfge8.L MGC145109, MGC75581 X. laevis Throughout NF stage 62 nerve optic nerve rac1.L mig5, p21-rac1, rac, tc-25, Xrac X. laevis Throughout NF stage 62 nerve optic nerve lrp1.S a2mr, apoer, apr, cd91, igfbp3r, lrp, tgfbr5 X. laevis Throughout NF stage 62 nerve optic nerve Image source: Published Larger Image Printer Friendly View

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