Xenbase Image ID: 149524

Fig. S1. Confirmation of selected array clones by RT-PCR analysis. Twenty-five percent of the array clones that resulted upregulated by Gata5 was validated by RT-PCR analysis, reasoning that this was a representative sample size. RT-PCR was performed on cDNA synthesized from ectodermal explants expressing Gata5. ODC was used as loading control and Pdx1 as positive control for Gata5 induction. The primers used for RT-PCR analysis are available at the http://xenopus.rockefeller.edu/. Image published in: Spagnoli FM and Brivanlou AH (2008) Copyright © 2008. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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